Composite
Part:BBa_K3286104:Design
Designed by: Robert Hooftman Group: iGEM19_Wageningen_UR (2019-10-14)
Expression cassette for small proteins in E. coli BL21DE3
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 493
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 191
Design Notes
Insert new fragments by amplifying the part with restriction enzyme sites and doing a ligation
Source
Parts amplified from plasmids present at our university
References
[1] V. K. Mutalik et al., “Precise and reliable gene expression via standard transcription and translation initiation elements,” Nat. Methods, vol. 10, no. 4, pp. 354–360, Apr. 2013.