Composite

Part:BBa_K3286104:Design

Designed by: Robert Hooftman   Group: iGEM19_Wageningen_UR   (2019-10-14)


Expression cassette for small proteins in E. coli BL21DE3


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 493
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 191


Design Notes

Insert new fragments by amplifying the part with restriction enzyme sites and doing a ligation


Source

Parts amplified from plasmids present at our university

References

[1] V. K. Mutalik et al., “Precise and reliable gene expression via standard transcription and translation initiation elements,” Nat. Methods, vol. 10, no. 4, pp. 354–360, Apr. 2013.